Serratia marcescens
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BIOL 240 visual lab guide BIOL 240 My home page

Visual Lab Study Guide

These pages are designed to help you interpret your lab results and to understand microbial physiology. The photographs on these pages cannot replace the experience gained from doing the experiments.

Use and Care of the Microscope Observing bacteria 1
Examination of Lliving Microorganisms Wet mount 2
Preparation of Smears Notes 3
Negative stain Stain 4
Gram Stain Interpreting a Gram stain 5
Acid Fast Staining Interpreting an acid-fast stain 6
Structural Stains Capsule stain
Endospore stain		 7
Morphologic Unknown Constructing a dichotomous key
 8
Microbes in the Environment Bacterial and fungal colonies
Nutrient broth		 9
Transfer of Bacteria: Aseptic Technique Culture media
Motility in a deep
Lactococcus 		10
Isolation of Bacteria by Dilution Techniques Pour plates
Find the pure culture
Streptomyces		 11
Special Media for Isolating Bacteria

MSA
EMB

12
Carbohydrate Catabolism Starch hydrolysis
OF medium		 13
Fermentation of Carbohydrates MR test
VP test
Fermentation tubes
Citrate		 14
Protein Catabolism, Part 1 Urea hydrolysis
Gelatin hydrolysis
Litmus milk		 15
Protein Catabolism, Part 2 Indole test
Peptone iron agar
MIO
Phenylalanine deaminase		 16
Respiration Oxidase test
Nitrate reduction
Catalase test		 17
Rapid Identification Methods IMViC
Enterotube
API
Differentiation of Enterobacteriaceae.pdf		 18
Oxygen and the Growth of Bacteria Thioglycollate 19
Determination of a Bacterial Growth Curve Sample graphs
Critical Thinking Qu. #2
Cell numbers and Abs.		 20
Other Influences on Microbial Growth
Biofilms 		Review quiz
In nutrient broth		 21
Physical Methods of Control: Heat
 Graph of sample results & class data
The Demonstration Plates		 22
Physical Methods of Control: Ultraviolet Radiation UV radiation 23
Chemical Methods of Control: Disinfectant and Antiseptics Sample results 24
Chemical Methods of Control: Antimicrobial Drugs Antibiosis
Kirby-Bauer Test		 25
Effectiveness of Hand Scrubbing Sample results 26
Control of Gene Expression Green Fluorescent Protein
Nitrate reductase
lac operon		 27
    28
Transformation of Bacteria Isolation of DNA
Transformation		 29
DNA Fingerprinting RFLPs 30
Genetic Engineering Recombinant DNA 31
Ames Test for Detecting Possible Chemical Carcinogens Ames Test 32
Unknown Identification and Bergey's Manual Differentiation of Enterobacteriaceae.pdf 33
Fungi: Yeasts Yeast 34
Fungi: Molds Aspergillus 35
    36
Protozoa Photos 37
Isolation of Bacteriophages Bacteriophage 38
Plant Viruses Tomato 39
Epidemiology Serratia
Epidemiology		 40
Koch's Postulates Koch's Postulates
Soft rot
Milky spore disease		 41
    42
Blood Group Determination Slide agglutination 43
Agglutination Reactions Sample results & Self-quiz 44
ELISA Technique Sample results 45
Bacteria of the Skin  Coagulase test
Mannitol salt agar
Tellurite glycine agar		 46
Bacteria of the Respiratory Tract Blood agar 47
Bacteria of the Mouth  MSB Agar 48
Bacteria of the Gastrointestinal Tract MacConkey agar
TSI slant
PEA		 49
Bacteria of the Urogenital Tract Pseudomonas
Neisseria gonorrhoeae		 50
Identification of an Unknown from a Clinical Sample The assignment
Differentiation of Enterobacteriaceae.pdf 		51
Microbes in Water: Multiple Tube Technique MPN Test
MUG agar		 52
Microbes in Water: Membrane Filter Techniques EMB agar 53
Microbes in Food: Contamination Standard Plate Count 54
Microbes Used in the Production of Foods Beer
Chocolate 		55
Microbes in Soil: The Nitrogen and Sulfur Cycles Winogradsky column 56
Microbes in Soil: Bioremediation Lipid hydrolysis
Enrichments		 57
Serial Dilutions About serial dilutions
Appendix B answers		 B