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Biology 230

Recombinant DNA and Transformation

(BIOL 230, Lab Experiment 19)

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Flow chart for the experiment | Bam and Hind digests | Restriction map answers

Using the maps below, answer the following questions about the gel:

1. Which restriction fragment of pAMP contains the amp resistance gene?

 

2. Which restriction fragment of pKAN contains the kan resistance gene?

 

3. Which restriction fragment is 784 bp?

 

4. Why are the pKAN fragments so close together?

Show me the answers.

What can you conclude from these plates? The original culture was ampsen-kansen E. coli.