• Sample preparation. Prepare an extract by grinding the appropriate
tissue in a few milliliters of solvent in a mortar and pestle. Possible
solvents are sterile water; methyl alcohol, ethyl alcohol, and acetone.
• Screening: antibacterial
• Serial dilutions
* Effect on bacterial/fungal growth in
* Bacteriocins (AMPs)
• Isolating active compounds
• Population growth
Plants to choose from:
We'll need to go on a walk to find these:
1 | 2
Farmed mushrooms (try a Japanese market)
Wild on campus…if it ever rains again
References to get started
1. Aldridge, S. “Marine Bioprospecting for Novel Drug.”
Genetic Engineering News 26(21):00-00, Dec 1 2006.
2. Akinjogunla et
al. "Antimicrobial potential of Nymphaea lotus (Nymphaeaceae)
against wound pathogens. " Journal of Medicinal Plants Research3(3):138-141,
3. Carlson, J. J., H. G. Douglas, and J. Robertson.. “Antibacterial
substances separated from plants.” Journal of Bacteriology
4. Cowan, M. M. “Plant Products as Antimicrobial Agents.”
Clinical Microbiology Reviews 12(4); 564-582, 1999.
5.Friedman M, Henika PR, Levin CE, Mandrell RE. “Antibacterial
activities of plant essential oils and their components against Escherichia
coli O157:H7 and Salmonella enterica in apple juice.”
Journal of Agricultural Food Chemistry 52(19):6042-6048, 2004..
6. Levy, S. R. "The challenge of antibiotic resistance." Scientific
American March 1998 pp. 48-53.