pelican
White Pelican with fish,
S. F. Bay
Photo©CLCase

Biology 230


Research Project

 

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Purpose
To identify and characterize antimicrobial from plants. Invasive plants disrupt many natural habitats. In the worst cases, invasive plants like mile-a-minute, cape ivy and kudzu ruthlessly choke out other plant life. This puts extreme pressure on native plants and animals, and threatened species may succumb to this pressure. Ultimately, invasive plants alter habitats and reduce biodiversity. Some of the plants on the list are invasives. In looking for new antimicrobials, we will see whether they have any redeeming qualities.

Possible procedures
• Sample preparation. Prepare an extract by grinding the appropriate tissue in a few milliliters of solvent in a mortar and pestle. Possible solvents are sterile water; methyl alcohol, ethyl alcohol, and acetone.
Screening: antibacterial or antifungal??
Serial dilutions
* MIC
* Effect on bacterial/fungal growth in food
Lysozyme
* Bacteriocins (AMPs)
Isolating active compounds
Population growth

Plants to choose from:
We'll need to go on a walk to find these:

Native
Rhamnus

Salt marsh natives
Grindelia stricta
Spartina foliosa
Distichlis spicata

Salicornia depressa
Field guides 1 | 2

Invasives
Cytisus
spp.
Delairea odorata
Pyracantha

 Fungi
Farmed mushrooms (try a Japanese market)
Wild on campus…if it ever rains again


Cultures available:
Aspergillus sp.
Escherichia coli
Micrococcus luteus
Salmonella enterica
Shigella sonnei
Staphylococcus aureus
Saccharomyces cerevisiae
Penicillium
sp.
Pseudomonas aeruginosa
Streptococcus pneumoniae
Streptococcus pyogenes
Streptococcus mutans

References to get started
1. Aldridge, S. “Marine Bioprospecting for Novel Drug.” Genetic Engineering News 26(21):00-00, Dec 1 2006.
2. Akinjogunla et al. "Antimicrobial potential of Nymphaea lotus (Nymphaeaceae) against wound pathogens. " Journal of Medicinal Plants Research3(3):138-141, 2009.
3. Carlson, J. J., H. G. Douglas, and J. Robertson.. “Antibacterial substances separated from plants.” Journal of Bacteriology 55(2):241-248, 1948.
4. Cowan, M. M. “Plant Products as Antimicrobial Agents.” Clinical Microbiology Reviews 12(4); 564-582, 1999. pdf
5.Friedman M, Henika PR, Levin CE, Mandrell RE. “Antibacterial activities of plant essential oils and their components against Escherichia coli O157:H7 and Salmonella enterica in apple juice.” Journal of Agricultural Food Chemistry 52(19):6042-6048, 2004..
6. Levy, S. R. "The challenge of antibiotic resistance." Scientific American March 1998 pp. 48-53.