Possible procedures
• Sample preparation. Prepare an extract by grinding the appropriate
tissue in a few milliliters of solvent in a mortar and pestle. Possible
solvents are sterile water; methyl alcohol, ethyl alcohol, and acetone.
• Screening: antibacterial
or antifungal??
• Serial dilutions
* MIC
* Effect on bacterial/fungal growth in
food
• Lysozyme
* Bacteriocins (AMPs)
• Isolating active compounds
• Population growth
|
Plants to choose from:
We'll need to go on a walk to find these: |
|
Native
Rhamnus
Salt
marsh natives
Grindelia stricta
Spartina foliosa
Distichlis spicata
Salicornia depressa
Field guides
1 | 2 |
Invasives
Cytisus spp.
Delairea odorata
Pyracantha
Fungi
Farmed mushrooms (try a Japanese market)
Wild on campus…if it ever rains again
|
Cultures available:
Aspergillus sp.
Escherichia coli
Micrococcus luteus
Salmonella enterica
Shigella sonnei
Staphylococcus aureus
Saccharomyces cerevisiae
Penicillium sp.
Pseudomonas aeruginosa
Streptococcus pneumoniae
Streptococcus pyogenes
Streptococcus mutans
References to get started
1. Aldridge, S. “Marine Bioprospecting for Novel Drug.”
Genetic Engineering News 26(21):00-00, Dec 1 2006.
2. Akinjogunla et
al. "Antimicrobial potential of Nymphaea lotus (Nymphaeaceae)
against wound pathogens. " Journal of Medicinal Plants Research3(3):138-141,
2009.
3. Carlson, J. J., H. G. Douglas, and J. Robertson.. “Antibacterial
substances separated from plants.” Journal of Bacteriology
55(2):241-248, 1948.
4. Cowan, M. M. “Plant Products as Antimicrobial Agents.”
Clinical Microbiology Reviews 12(4); 564-582, 1999.
pdf
5.Friedman M, Henika PR, Levin CE, Mandrell RE. “Antibacterial
activities of plant essential oils and their components against Escherichia
coli O157:H7 and Salmonella enterica in apple juice.”
Journal of Agricultural Food Chemistry 52(19):6042-6048, 2004..
6. Levy, S. R. "The challenge of antibiotic resistance." Scientific
American March 1998 pp. 48-53.