Note Before beginning, you will need to know about the •solubility of tergitol. •concentrations you will test in order to request a stock solution (starting concentration from which you will make dilutions).

Materials available
Live Cultures
Paramecium
Euglena
Real ecosystem

Glassware/plasticware
Cell well plates
Small tubes ( 6 mm x 50 mm), (10 mm x 75 mm)
Petri slides
Petri plates (47 mm, 25 mm)
Pipettes, micropipettes

Solutions
Culture media
Sterile distilled water, 99-ml aliquots & 9-ml aliquots
Sterile isotonic saline, 99-ml aliquots & 9-ml aliquots
Tergitol: you need to determine your concentration

Equipment
Growth chamber
Inverted microscope
Microscope

Procedures
•Growth Curves
•Serial dilutions
• LC₅₀