Procedures
• Sample preparation. Prepare an extract by grinding the appropriate tissue in a few milliliters of solvent in a mortar and pestle. Possible solvents are sterile water; methyl alcohol, ethyl alcohol, and acetone.
• Screening
• Serial dilutions
* MIC
• Lysozyme
* Bacteriocins (AMPs)
• Isolating active compounds
• Bacterial numbers
• Population growth

Plants/algae to chose from
Heteromeles
Ceanothus
Aesculus
Arctostaphylos
Rhodophyta
Phaeophyta

Cultures available:
Aspergillus sp.
Escherichia coli
Micrococcus luteus
Salmonella enterica
Shigella sonnei
Staphylococcus aureus
Saccharomyces cerevisiae
Penicillium sp.
Pseudomonas aeruginosa
Streptococcus pneumoniae
Streptococcus pyogenes
Streptococcus mutans

References
1. Aldridge, S. “Marine Bioprospecting for Novel Drug.” Genetic Engineering News 26(21):00-00, Dec 1 2006.
2. Carlson, J. J., H. G. Douglas, and J. Robertson. 1948. “Antibacterial substances separated from plants.” Journal of Bacteriology 55(2):241-248.
3. Cowan, M. M. 1999. “Plant Products as Antimicrobial Agents.” Clinical Microbiology Reviews 12(4); 564-582. pdf
4.Friedman M, Henika PR, Levin CE, Mandrell RE. 2004 “Antibacterial activities of plant essential oils and their components against Escherichia coli O157:H7 and Salmonella enterica in apple juice.” Journal of Agricultural Food Chemistry 52(19):6042-6048.
5. Levy, S. R. "The challenge of antibiotic resistance." Scientific American March 1998 pp. 48-53.